Journal: International Journal of Medical Sciences

Article Title: Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis

doi:

Figure Lengend Snippet: PARP is active and necessary for efficient apoptosis in NS1-transfected cells. A. Immunoprecipitated GFP/NS1 protein was poly(ADP ribose)ylated, as shown by a band at 100 kd on the blot probed with anti-poly ADP ribose (PAR, left blot) antibodies. The blots were stripped and reprobed with anti-GFP (right), showing that PAR colocalizes with the GFP/NS1 fusion protein. GFP alone is not ribosylated, as evidenced by the lack of a PAR band corresponding to GFP at 37 kD. Blots shown are representative of three independent experiments. B. PARP activity is necessary for optimal NS1-induced apoptosis. Addition of the PARP inhibitor 5-aminoisoquinolinone (5AIQ) to GFP/NS1 expressing HepG2 cells reduced apoptosis by 57% ( p <0.003). Addition of 5-aminoisoquinolinone had no effect on the GFP transfected cells. N=3, error bars indicate the range of values.

Article Snippet: 25 μl of anti-GFP polyclonal antibody (Rockland, Gilbertsville, PA) were added and the mixture was allowed to bind for 14 hours at 4 o C in an end-over end rotator.

Techniques: Transfection, Immunoprecipitation, Activity Assay, Expressing

Journal: Antibody therapeutics

Article Title: First site-specific conjugation method for native goat IgG antibodies via glycan remodeling at the conserved Fc region.

doi: 10.1093/abt/tbae014

Figure Lengend Snippet: Figure 7. Detection of GFP by the native and conjugated goat anti-GFP IgGs as primary antibodies in capillary western blot analysis. Lanes 1–2: AF 488 conjugate. Lanes 3–4: native goat IgG. Lanes 5–6: PEG conjugate.

Article Snippet: Goat polyclonal anti-GFP IgG antibodies (600-101-215) and GFP (000-001-215) were from Rockland Immunochemicals (Limerick, PA, USA).

Techniques: Western Blot